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[软件使用] bowtie遇错,求帮助

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  • TA的每日心情
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    2019.6.12 09:43
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    发表于 2019.4.8 11:31:00 | 显示全部楼层 |阅读模式
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    使用bowtie时遇到错误不知道如何解决,求高手指教
    bowtie2-build GCA_900519105.1_iwgsc_refseqv1.0_genomic.fna wheat
    Settings:
      Output files: "wheat.*.bt2l"
      Line rate: 7 (line is 128 bytes)
      Lines per side: 1 (side is 128 bytes)
      Offset rate: 4 (one in 16)
      FTable chars: 10
      Strings: unpacked
      Max bucket size: default
      Max bucket size, sqrt multiplier: default
      Max bucket size, len divisor: 4
      Difference-cover sample period: 1024
      Endianness: little
      Actual local endianness: little
      Sanity checking: disabled
      Assertions: disabled
      Random seed: 0
      Sizeofs: void*:8, int:4, long:8, size_t:8
    Input files DNA, FASTA:
      GCA_900519105.1_iwgsc_refseqv1.0_genomic.fna
    Building a LARGE index
    Reading reference sizes
      Time reading reference sizes: 00:02:17
    Calculating joined length
    Writing header
    Reserving space for joined string
    Could not allocate space for a joined string of 14271578887 elements.
    Switching to a packed string representation.
    Building a LARGE index
    Reading reference sizes
      Time reading reference sizes: 00:02:18
    Calculating joined length
    Writing header
    Reserving space for joined string
    Could not allocate space for a joined string of 14271578887 elements.
    Please try running bowtie-build on a computer with more memory.
    Total time for call to driver() for forward index: 00:04:35
    Error: Encountered internal Bowtie 2 exception (#1)
    Command: bowtie2-build --wrapper basic-0 GCA_900519105.1_iwgsc_refseqv1.0_genomic.fna wheat
    Deleting "wheat.3.bt2l" file written during aborted indexing attempt.
    Deleting "wheat.4.bt2l" file written during aborted indexing attempt.
    Deleting "wheat.1.bt2l" file written during aborted indexing attempt.
    Deleting "wheat.2.bt2l" file written during aborted indexing attempt.
    Deleting "wheat.3.bt2l" file written during aborted indexing attempt.
    Deleting "wheat.4.bt2l" file written during aborted indexing attempt.
    Deleting "wheat.1.bt2l" file written during aborted indexing attempt.
    Deleting "wheat.2.bt2l" file written during aborted indexing attempt.




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     楼主| 发表于 2019.4.8 15:23:13 | 显示全部楼层
    Error: Encountered internal Bowtie 2 exception (#1)
    这个错误怎么解决啊?搜索了很久没找到解决方法。
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    发表于 2019.4.9 13:48:19 | 显示全部楼层
    Please try running bowtie-build on a computer with more memory.
    很明显了啊
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