More powerful tools, please visit (Dynamic principal coordinate analysis)

Select a file   Example

Select a file   Example

Select a file    Example

Tool machinery:

(1)If importing an OTU table, the tool will first normalize each OTU in the relative abundance, then calculate sample similarity distance of Bray-Curtis by R. Finally, PCoA is performed in R and plotted using ggplot2 software.

(2)If importing similarity distance matrix (e.g. Unifrac distance), the PCoA tool will calculate and plot a chart directly from the input matrix.

PCoA (principal co-ordinates analysis) is a means of visualizing the level of similarity of individual cases of a dataset.


(1) The input file can be abundance table(OTU,species,funtion abundance table)or similarity distance matrix

(2)Grouping file is optional. The tool supports no more than 2 grouping information in a single sample.

(3) The input file should be a tab-delimited “.txt” file


(1)Dot size:Dot size: define the dots size(the default value is 3)

(2)Group name:define the group name

(3)Dot transparency:define the dots transparency if case of overlapping of dots


PCoA_sites.xls:document dot sites in a PCoA plot.

PCoA_eig.xls:document the sum of all eigenvalues and importance of each axis.

pcoa_plot.png/pdf: PCoA plot of PCo1-PCo2, PCo1-PCo3 and PCo2-PCo3.


Abundance table    Grouping file1    Grouping file2


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