Swiss-Prot annotation





choosefile  example






Function:

UniProtKB/Swiss-Prot is the manually annotated and reviewed section of the UniProt Knowledgebase (UniProtKB). It is a high quality annotated and non-redundant protein sequence database, which brings together experimental results, computed features and scientific conclusions.

We use DIAMOND software to align the query sequences to the Swiss-Prot protein database to find the proteins with highest sequence similarity.


Input

paste or upload a nucleic or protein sequence in FASTA format.


Output:

① alignment result table

② alignment statistic pie chart ( alignment number pie chart and E-value distribution pie chart)




example



Output:

① alignment result table


Headers:

Query_id                               the ID of the query sequence

Subject_id                   the ID of the subject sequence

Identity(%)                 the alignment similarity (percentage)

Align_length                alignment length

Mis_match                 number of mismatch

Gap                            number of Gap

Query_start               the start site of the aligned query sequence

Query_end                the end site of the aligned query sequence

Subject_start              the start site of the aligned subject sequence

Subject_end               the end site of the aligned subject sequence

E_value                   E value of alignment (the smaller the E value, the more the reliability)

Score                     the score of alignment (the higher the score, the more the reliability)

Subject_annotation          description of Swiss-Prot subject sequence


② alignment number pie chart

We count up the aligned sequences number and show the result with a pie chart.


③ E-value distribution pie chart

E value is the expected value of the alignment. The smaller the E value, the more the reliability. We divided the E value into five ranges and show the number with a pie chart.