t-test tool





Select a file    Example


Select a file    Example


Select a file    Example










Histogram    Volcano plot    Heat map







Function:
The t-test tool is used to determine if two groups of data are significantly different from each other.The t-test is any statistical hypothesis test in which the test statistic follows a Student's t-distribution under the null hypothesis.


Input:

Attention:Input tab delimited text files with headers. If your data is not TAB delimited, you can use Excel to convert it.

(1) Different analysis table file:Input a tab delimited text file with headers. The first row is sample names and the first column is variable name. Values in the table are variable value.Each group should have 3 samples at least, and multiple groups of samples should be compared in pairs

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(2) Comparison file:Input a tab delimited text file where a row represents a comparison with the second column versus the first column.

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(3) Grouping file:Input a tab delimited text file where sample names in the first column and group names in the second column

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Parameters:

(1) t-test type: Unpaired t-test or paired t-test. Paired samples t-tests typically consist of a sample of matched pairs of similar units, or one group of units that has been tested twice.。

(2) Test value: you can choose P value or Q value for test value. Q value (false discovery rate, FDR) is calculated by FDR correction of P value.

(3) Threshold for P value or Q value: Normally, the threshold for differential expression analysis is 0.05. You can also set the threshold according to your experiment, like 0.01 or 0.001.

(4) Threshold of fold change: The default of is 1, which means you don't think about fold change.


Graph:The default is histogram, the optional graphs are volcano plot and heat map.

diff_stat.xls: statistical table of differential variable numbers
diff_stat.pdf/png: differential variable histogram between comparison groups (vector/scalar figure)
A-vs-B.Ttest.all. xls: Differentially variable values table
A-vs-B. Ttest.filter.xls: Significantly differentially variable values table
A-vs-B.volcano.pdf/png: Differentially variable values volcano plot (vector/scalar figure)
A-vs-B.heatmap.pdf/png: Differentially variable values volcano plot (vector/scalar figure)




Example: Different analysis table file    Comparison file    Grouping file   



Result graph:(The default is histogram, the optional graphs are volcano plot and heat map)

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some result graphs:

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